Immunoactive Effect of Gryllus bimaculatus on Macrophage Cells under Different Extraction Temperatures

In this study, the immunoactive effect of Gryllus bimaculatus water extract (GBWE) on macrophages was investigated under various extraction temperature conditions. The study found that GBWE did not induce cytotoxicity in RAW 264.7 cells within tested concentration range. Treatment with different conditions resulted an increase cytokine secretion and nitric oxide production treated cells. Furthermore, treatment increased expression surface molecules, GBWE-treated showed phosphorylation mitogen-activated protein kinases nuclear factor-kappa B translocation. experimental results suggest exhibits immune-enhancing effects macrophages. Notably, these were observed even extracted at low temperatures (5°C 25°C) without exposure to high (75°C) typically used for extraction. These findings has potential be as alternative source functional food material immunostimulatory properties.